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<h1 class="ltx_title ltx_title_section">Removing duplicates</h1>

<div id="Sx1.p1" class="ltx_para">
<p class="ltx_p">Repeated localizations of single molecules in one frame may occur
due to overlapping fitting sub-regions when using the <a href="../estimators/ui/CrowdedField.html" title="" class="ltx_ref">multiple-emitter analysis approach</a>.
To deal with the problem, ThunderSTORM uses a post-processing method
suggested by Huang et al. <cite class="ltx_cite">[<a href="#bib.bib15" title="Simultaneous multiple-emitter fitting for single molecule super-resolution imaging" class="ltx_ref">1</a>]</cite>. Here molecules with
a mutual distance smaller than their localization uncertainty are
grouped together, and in each group, only the molecule with the smallest
localization uncertainty is kept. The other molecules are rejected.
ThunderSTORM generalizes this algorithm by allowing users to specify
the distance threshold for grouping the molecules as a mathematical
expression. A different distance threshold can be applied to individual
molecules if the mathematical expression results in a vector. The
same distance threshold is applied to all molecules if the result
is a scalar.</p>
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<div id="Sx1.SSx1" class="ltx_subsection">
<h2 class="ltx_title ltx_title_subsection">See also</h2>

<div id="Sx1.SSx1.p1" class="ltx_para">
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<li id="I1.i1" class="ltx_item" style="list-style-type:none;">
<span class="ltx_tag ltx_tag_itemize">•</span> 
<div id="I1.i1.p1" class="ltx_para">
<p class="ltx_p"><a href="Postprocessing.html" title="" class="ltx_ref">Post-processing analysis</a></p>
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<h1 class="ltx_title ltx_title_bibliography">References</h1>

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<li id="bib.bib15" class="ltx_bibitem ltx_bib_article">
<span class="ltx_bibtag ltx_bib_key ltx_role_refnum">[1]</span>
<span class="ltx_bibblock"><span class="ltx_text ltx_bib_author">F. Huang, S. L. Schwartz, J. M. Byars and K. A. Lidke</span><span class="ltx_text ltx_bib_year">(2011)</span>
</span>
<span class="ltx_bibblock"><span class="ltx_text ltx_bib_title">Simultaneous multiple-emitter fitting for single molecule super-resolution imaging</span>,
</span>
<span class="ltx_bibblock"><span class="ltx_text ltx_bib_journal">Biomedical Optics Express</span> <span class="ltx_text ltx_bib_volume">2</span> (<span class="ltx_text ltx_bib_number">5</span>), <span class="ltx_text ltx_bib_pages"> pp. 1377–93</span>.
</span>
<span class="ltx_bibblock">External Links: <span class="ltx_text ltx_bib_links"><a href="http://dx.doi.org/10.1364/BOE.2.001377" title="" class="ltx_ref doi ltx_bib_external">Document</a></span>.
</span>
<span class="ltx_bibblock ltx_bib_cited">Cited by: <a href="#Sx1.p1" title="Removing duplicates" class="ltx_ref"><span class="ltx_text ltx_ref_title">Removing duplicates</span></a>.
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